SNP Genotyping, DNA Marker, PCR, Mutation, Microsatellite (STR) Analysis

For almost all projects which include use of DNA markers and genetic variability, we are able to offer the appropriate technical platform and scientific expertise. With our certified laboratory, we are one of the international leaders in the field of applied genetics. Typically, our service for genotype, SNP, mutation and DNA marker analysis is requested in the following areas:

• Forensic DNA analysis
• Clinical studies
• Research and Diagnostic
• Plant breeding
• Livestock breeding (agriculture and pets)
• Population genetics
• Academic projects with animals, plants and micro-organisms
• Industrial Biotechnology
• Protection of endangered species
• Identification of ingredients in food and products
• Food and product authenticity (species and breed identification)

Our offer ranges from comprehensive consulting and complete realisation of projects down to the directed use of technology platforms through our customers. Main focal points are:

• DNA extraction from different tissues and materials up to ten thousands of samples
  
  
• Genotyping of SNPs and INDEL polymorphisms
  
  
• Genotyping of Short Tandem Repeats (STR) and microsatellites by fragment length analysis
  
  
• Quantitative PCR / Real-time PCR and analysis of melting curves with ABI TaqMan or Roche LightCycler
  
  
• DNA Sequencing from single samples up to high throughput with more than 500 capillaries
  
  
• 'Next Generation DNA Sequencing' up to 100.000.000 bases per day
  
  
• Restriction fragment length polymorphism (RFLP)
  
  
• Amplified fragment length polymorphism (AFLP)

Contact our experts for a personal consultancy!
Dr. Katrin Juling	tel.: +49 8092 / 8289-214	email: juling@medigenomix.de
Dr. Jens Wiehler	tel.: +49 8092 / 8289-250	email: wiehler@medigenomix.de
Dr. Rainer Schubbert	tel.: +49 8092 / 8289-222	email: schubbert@medigenomix.de
Or you may also use our CONTACT FORM. We have worldwide experienced distribution partners. Ask us for your local contact.

DNA extraction from different tissues and materials up to ten thousands of samples

At the beginning of all analyses DNA or RNA needs to be extracted. Here we count, especially with sensitive samples, on our long-lasting experience in forensic DNA trace analysis. Single analysis of food samples or parallel preparation of ten thousands of blood and tissue samples, our DNA know how sets standards. Our well-trained laboratory staffs work according to a certified quality management system. Automated platforms and pipetting robots facilitate the high-throughput analysis and enable a consistent quality standard.

Courtesy of Sequenom®

Genotyping of SNPs and INDEL polymorphisms

For High Throughput SNP genotyping we recommend the SEQUENOM MassARRAY^® iPlex Gold System, which combines the simplicity of a single base extension reaction with the sensitivity and precision of MALDI-TOF mass spectroscopy. The advantages of the system are obvious: A high level of automation, a very fast turnaround time, reproducible and understandable results and highest exactness.

Typical applications: High-throughput genotyping of one to hundred SNPs per sample, e.g., fine mapping of gene loci, haplotyping, high priority SNP genotyping from whole genome studies, population genetics, association studies (also with pooled DNA and mutations with low frequencies), validation studies, parentage and pedigree testing, traceability, genomic selection, etc.

Potential for Forensic DNA analysis please see, for example, some of our recently published R&D results (pdf, 800 KB) >>

Genotyping of Short Tandem Repeats (STR) or microsatellites by fragment length analysis

Genotyping of microsatellites by fragment length analysis of fluorescent PCR products with Capillary Electrophoresis. Expect fast and accurate services with more than 40 thermal cyclers and electrophoretic separation on ABI 3130 and ABI 3100 automatic sequencers.

Typical applications: DNA forensics, parentage testing and genealogy of human, parentage and pedigree testing of animals, population genetics, QTL studies, etc.

Quantitative PCR / Real-time PCR and melting curve analysis with ABI TaqMan or Roche Light Cycler

Precise, sensitive and fast quantitative measurement of target DNA or RNA. We offer these analyses in plate format up to 2 x 384 samples.

Typical applications: Projects with a small number of SNPs or problematic SNPs and preliminary studies, expression analysis, clinical or diagnostic studies (human and veterinary). Identification of species, even traces, in food, products, and forensics. LightCycler and melting curves analyses are often requested in clinical and diagnostic areas.
For special applications, i.e. DNA and RNA quantification in food and products or identification of species and breeds we offer with an Agilent 2100 bio-analyser the 'lab-on-the-chip' technology. Epigenetic studies and methylation studies, e.g. with the SEQUENOM MassARRAY^® EpiTyper system will be discussed on request.

Courtesy of Sequenom®

DNA sequencing from single samples up to high throughput with more than 500 capillaries

Sanger DNA sequencing with fluorescent chain terminating nucleotides and separation of the fragments with capillary electrophoresis. More than 10 modern ABI capillary sequencers equipped with more than 500 capillaries allow quick and reliable sequencing or genotyping.

Typical applications: If you need to be quick without establishing other methods, with low sample numbers or if comprehensive sequence information needs to be covered. I.e. collection of sequence variability in populations, or preliminary studies in human or veterinary medicine. Also for genotyping of problematic DNA-markers or mitochondrial DNA (parentage testing, population genetics, species identification). Our high-throughput genotyping technologies can be established and optimised based on the derived sequence information.

'Next Generation DNA Sequencing' up to 100,000,000 bases in a day

Now, a real high throughput sequencing analysis is available: with each of our both Roche Genome FLX Sequencers supported by the 454 Sequencing system we can produce millions over 400,000 reads equalling over 100,000,000 basepairs! Parallelization, state-of-the-art image processing, and unique data analysis allow high quality sequencing at cyber speed.

Typical applications: Identification of genetic variation between individuals, population and species. Population genetics, identification of DNA markers and QTL for animal and plant breeding. Identification of SNPs and INDEL polymorphisms, medical research, analysis of splice variants, etc. Re-sequencing (medical R&D, diagnostics, animal breeding, cancer research, inherited diseases, infectious diseases). De novo sequencing, BAC sequencing, whole genome sequencing, epigenetics, metagenetics, analysis of splice variants.

RFLP (Restriction fragment length polymorphism)
AFLP (Amplified fragment length polymorphism)

We offer a wide range of markers for discrimination of closely related species, breeds or variants. Also usable for studies based on phylogenetic differences or gene mapping. This genotyping technique finds mutations by the input of restrictions enzyme. The DNA fingerprint can be used to discriminate individuals or to show closely relations between individuals. We offer this method in plate format.

Typical applications: Animal and plant breeding, evaluation of botanical variety, population analysis, breed or species discrimination, QTL analyses, gene mapping.

Contact our experts for a personal consultancy!
Dr. Katrin Juling	tel.: +49 8092 / 8289-214	email: juling@medigenomix.de
Dr. Jens Wiehler	tel.: +49 8092 / 8289-250	email: wiehler@medigenomix.de
Dr. Rainer Schubbert	tel.: +49 8092 / 8289-222	email: schubbert@medigenomix.de
Or you may also use our CONTACT FORM. We have worldwide experienced distribution partners. Ask us for your local contact.